Polymorphic residues in HLA-B that mediate HIV control distinctly modulate peptide interactions with both TCR and KIR molecules.

ABSTRACT: Immunogenetic studies have shown that specific HLA-B residues (67, 70, 97, and 156) mediate the impact of HLA class I on HIV infection, but the molecular basis is not well understood. Here we evaluate the function of these residues within the protective HLA-B^∗5701 allele. While mutation of Met67, Ser70, and Leu156 disrupt CD8⁺ T cell recognition, substitution of Val97 had no significant impact. Thermal denaturation of HLA-B^∗5701-peptide complexes revealed that Met67 and Leu156 maintain HLA-peptide stability, while Ser70 and Leu156 facilitate T cell receptor (TCR) interactions. Analyses of existing structures and structural models suggested that Val97 mediates HLA-peptide binding to inhibitory KIR3DL1 molecules, which was confirmed by experimental assays. Th

SUBMITTER: Tano-Menka R 

PROVIDER: S-EPMC11329236 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature