Sumo-regulatory SENP2 controls the homeostatic squamous mitosis-differentiation checkpoint.

ABSTRACT: Squamous or epidermoid cancer arises in stratified epithelia but also is frequent in the non-epidermoid epithelium of the lung by unclear mechanisms. A poorly studied mitotic checkpoint drives epithelial cells bearing irreparable genetic damage into epidermoid differentiation. We performed an RNA-sequencing gene search to target unknown regulators of this response and selected the SUMO regulatory protein SENP2. Alterations of SENP2 expression have been associated with some types of cancer. We found the protein to be strongly localised to mitotic spindles of freshly isolated human epidermal cells. Primary cells rapidly differentiated after silencing SENP2 with specific shRNAs. Loss of SENP2 produced in synchronised epithelial cells delays in mitotic entry and exit and defects in chromosomal

SUBMITTER: Galan-Vidal J 

PROVIDER: S-EPMC11329632 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature