Targeted DNA-seq and RNA-seq of Reference Samples with Short-read and Long-read Sequencing.

ABSTRACT: Next-generation sequencing (NGS) has revolutionized genomic research by enabling high-throughput, cost-effective genome and transcriptome sequencing accelerating personalized medicine for complex diseases, including cancer. Whole genome/transcriptome sequencing (WGS/WTS) provides comprehensive insights, while targeted sequencing is more cost-effective and sensitive. In comparison to short-read sequencing, which still dominates the field due to high speed and cost-effectiveness, long-read sequencing can overcome alignment limitations and better discriminate similar sequences from alternative transcripts or repetitive regions. Hybrid sequencing combines the best strengths of different technologies for a more comprehensive view of genomic/transcriptomic variations. Understanding each technolo

SUBMITTER: Gong B 

PROVIDER: S-EPMC11329654 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature