Mechanistic insights into condensate formation of human liver-type phosphofructokinase by stochastic modeling approaches.

ABSTRACT: Human liver-type phosphofructokinase 1 (PFKL) has been shown to regulate glucose flux as a scaffolder arranging glycolytic and gluconeogenic enzymes into a multienzyme metabolic condensate, the glucosome. However, it has remained elusive of how phase separation of PFKL is governed and initiates glucosome formation in living cells, thus hampering to understand a mechanism of glucosome formation and its functional contribution to human cells. In this work, we developed a stochastic model in silico using the principle of Langevin dynamics to investigate how biological properties of PFKL contribute to the condensate formation. The significance of an intermolecular interaction between PFKLs, an effective concentration of PFKL at a region of interest, and its own self-assembled filaments in form

SUBMITTER: Kang HW 

PROVIDER: S-EPMC11329711 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature