Interleukin-1β polarization in M1 macrophage mediates myocardial fibrosis in diabetes.

ABSTRACT:

Background

Diabetes is a global health problem whose common complication is diabetic cardiomyopathy, characterized by chronic inflammation of the heart muscle. Macrophages are the main white blood cells found in the resting heart. Therefore, we investigated the underling mechanism of macrophage on myocardial fibrosis in diabetes.

Methods

Here, echocardiography was utilized to evaluate cardiac function, and the degree of myocardial fibrosis was assessed using Masson's trichrome staining, followed by single-cell RNA sequencing (scRNA-seq) to analyze the phenotype, function, developmental trajectory, and interactions between immune cells, endothelial cells (ECs), and fibroblasts (FBs) in the hearts of db/db mice at different stages of diabetes. Macrophages and cardiac fibroblas

SUBMITTER: Guo W 

PROVIDER: S-EPMC11330059 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature