The role of DNA polymerase I in tolerating single-strand breaks generated at clustered DNA damage in Escherichia coli.

ABSTRACT: Clustered DNA damage, when multiple lesions are generated in close proximity, has various biological consequences, including cell death, chromosome aberrations, and mutations. It is generally perceived as a hallmark of ionizing radiation. The enhanced mutagenic potential of lesions within a cluster has been suggested to result, at least in part, from the selection of the strand with the mutagenic lesion as the preferred template strand, and that this process is relevant to the tolerance of persistent single-strand breaks generated during an attempted repair. Using a plasmid-based assay in Escherichia coli, we examined how the strand bias is affected in mutant strains deficient in different DNA polymerase I activities. Our study revealed that the strand-displacement and 5'-flap endonuclease

SUBMITTER: Shikazono N 

PROVIDER: S-EPMC11330960 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature