Dataset Information

M⁶A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity.

ABSTRACT: Plants employ distinct mechanisms to respond to environmental changes. Modification of mRNA by N⁶-methyladenosine (m⁶A), known to affect the fate of mRNA, may be one such mechanism to reprogram mRNA processing and translatability upon stress. However, it is difficult to distinguish a direct role from a pleiotropic effect for this modification due to its prevalence in RNA. Through characterization of the transient knockdown-mutants of m⁶A writer components and mutants of specific m⁶A readers, we demonstrate the essential role that m⁶A plays in basal resistance and pattern-triggered immunity (PTI). A global m⁶A profiling of mock and PTI-induced Arabidopsis plants as well as formaldehyde fixation and cross-linking immunoprecipitation-sequencing of the m⁶A reader, EVOLUTIONARILY CONSERVED C-TERMINAL REGION2 (ECT2) showed that while dynamic changes in m⁶A modification and binding by ECT2 were detected upon PTI induction, most of the m⁶A sites and their association with ECT2 remained static. Interestingly, RNA degradation assay identified a dual role of m⁶A in stabilizing the overall transcriptome while facilitating rapid turnover of immune-induced mRNAs during PTI. Moreover, polysome profiling showed that m⁶A enhances immune-associated translation by binding to the ECT2/3/4 readers. We propose that m⁶A plays a positive role in plant immunity by destabilizing defense mRNAs while enhancing their translation efficiency to create a transient surge in the production of defense proteins.

SUBMITTER: Chen T

PROVIDER: S-EPMC11331096 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

m6A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity.

Chen Tianyuan T Greene George H GH Motley Jonathan J Mwimba Musoki M Luo Guan-Zheng GZ Xu Guoyong G Karapetyan Sargis S Xiang Yezi Y Liu Chang C He Chuan C Dong Xinnian X

Proceedings of the National Academy of Sciences of the United States of America 20240808 33

Plants employ distinct mechanisms to respond to environmental changes. Modification of mRNA by N 6-methyladenosine (m6A), known to affect the fate of mRNA, may be one such mechanism to reprogram mRNA processing and translatability upon stress. However, it is difficult to distinguish a direct role from a pleiotropic effect for this modification due to its prevalence in RNA. Through characterization of the transient knockdown-mutants of m6A writer components ...[more]

PMID: 39116132

Similar Datasets

Project description:BackgroundPoly C Binding Protein 1 (PCBP1) is an RNA-binding protein that binds and regulates translational activity of subsets of cellular mRNAs. Depletion of PCBP1 is implicated in various carcinomas, but the underlying mechanism in tumorigenesis remains elusive.MethodsWe performed a transcriptome-wide screen to identify novel bounding mRNA of PCBP1. The bind regions between PCBP1 with target mRNA were investigated by using point mutation and luciferase assay. Cell proliferation, cell cycle, tumorigenesis and cell apoptosis were also evaluated in ovary and colon cancer cell lines. The mechanism that PCBP1 affects p27 was analyzed by mRNA stability and ribosome profiling assays. We analyzed PCBP1 and p27 expression in ovary, colon and renal tumor samples and adjacent non-tumor tissues using RT-PCR, Western Blotting and immunohistochemistry. The prognostic significance of PCBP1 and p27 also analyzed using online databases.ResultsWe identified cell cycle inhibitor p27Kip1 (p27) as a novel PCBP1-bound transcript. We then demonstrated that binding of PCBP1 to p27 3'UTR via its KH1 domain mainly stabilizes p27 mRNA, while enhances its translation to fuel p27 expression, prior to p27 protein degradation. The upregulated p27 consequently inhibits cell proliferation, cell cycle progression and tumorigenesis, whereas promotes cell apoptosis under paclitaxel treatment. Conversely, knockdown of PCBP1 in turn compromises p27 mRNA stability, leading to lower p27 level and tumorigenesis in vivo. Moreover, forced depletion of p27 counteracts the tumor suppressive ability of PCBP1 in the same PCBP1 over-expressing cells. Physiologically, we showed that decreases of both p27 mRNA and its protein expressions are well correlated to PCBP1 depletion in ovary, colon and renal tumor samples, independent of the p27 ubiquitin ligase Skp2 level. Correlation of PCBP1 with p27 is also found in the tamoxifen, doxorubincin and lapatinib resistant breast cancer cells of GEO database.ConclusionOur results thereby indicate that loss of PCBP1 expression firstly attenuates p27 expression at post-transcriptional level, and subsequently promotes carcinogenesis. PCBP1 could be used as a diagnostic marker to cancer patients.

Dataset Information

M⁶A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity.

Publications

m<sup>6</sup>A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

M6A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity.

Publications

m<sup>6</sup>A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

M⁶A modification plays an integral role in mRNA stability and translation during pattern-triggered immunity.