Single-particle rotational microrheology enables pathological staging of macrophage foaming and antiatherosclerotic studies.

ABSTRACT: The formation of macrophage-derived foam cells has been recognized as the pathological hallmark of atherosclerotic diseases. However, the pathological evolution dynamics and underlying regulatory mechanisms remain largely unknown. Herein, we introduce a single-particle rotational microrheology method for pathological staging of macrophage foaming and antiatherosclerotic explorations by probing the dynamic changes of lysosomal viscous feature over the pathological evolution progression. The principle of this method involves continuous monitoring of out-of-plane rotation-caused scattering brightness fluctuations of the gold nanorod (AuNR) probe-based microrheometer and subsequent determination of rotational relaxation time to analyze the viscous feature in macrophage lysosomes. With this met

SUBMITTER: Shang J 

PROVIDER: S-EPMC11331104 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature