T6SS nuclease effectors in Pseudomonas syringae act as potent antimicrobials in interbacterial competition.

ABSTRACT: Type VI secretion system (T6SS) is a potent weapon employed by various Pseudomonas species to compete with neighboring microorganisms for limited nutrients and ecological niches. However, the involvement of T6SS effectors in interbacterial competition within the phytopathogen Pseudomonas syringae remains unknown. In this study, we examined two T6SS clusters in a wild-type P. syringae MB03 and verified the involvement of one cluster, namely, T6SS-1, in interbacterial competition. Additionally, our results showed that two T6SS DNase effectors, specifically Tde1 and Tde4, effectively outcompeted antagonistic bacteria, with Tde4 playing a prominent role. Furthermore, we found several cognate immunity proteins, including Tde1i^a, Tde1i^b, and Tde4i, whic

SUBMITTER: Yu X 

PROVIDER: S-EPMC11332151 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature