OsALKBH9-mediated m⁶A demethylation regulates tapetal PCD and pollen exine accumulation in rice.

ABSTRACT: The N⁶-methyladenosine (m⁶A) mRNA modification is crucial for plant development and stress responses. In rice, the male sterility resulting from the deficiency of OsFIP37, a core component of m⁶A methyltransferase complex, emphasizes the significant role of m⁶A in male fertility. m⁶A is reversible and can be removed by m⁶A demethylases. However, whether mRNA m⁶A demethylase regulates male fertility in rice has remained unknown. Here, we identify the mRNA m⁶A demethylase OsALKBH9 and demonstrate its involvement in male fertility regulation. Knockout of OsALKBH9 causes male sterility, dependent on its m⁶A demethylation activity. Cytological analysis reveals defective tapetal programmed cell dea

SUBMITTER: Tang J 

PROVIDER: S-EPMC11332222 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature