The trophoblast surface becomes refractory to adhesion by congenitally transmitted Toxoplasma gondii and Listeria monocytogenes during cytotrophoblast to syncytiotrophoblast development.

ABSTRACT: The placenta is a critical barrier against viral, bacterial, and eukaryotic pathogens. For most teratogenic pathogens, the precise molecular mechanisms of placental resistance are still being unraveled. Given the importance of understanding these mechanisms and challenges in replicating trophoblast-pathogen interactions using in vitro models, we tested an existing stem-cell-derived model of trophoblast development for its relevance to infection with Toxoplasma gondii. We grew human trophoblast stem cells (TS^CT) under conditions leading to either syncytiotrophoblast (TS^SYN) or cytotrophoblast (TS^CYT) and infected them with T. gondii. We evaluated T. gondii proliferation and invasion, cell ultrastructure, as well as for transcripto

SUBMITTER: da Silva RJ 

PROVIDER: S-EPMC11332349 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature