PLCγ1 deficiency in chondrocytes accelerates the age-related changes in articular cartilage and subchondral bone.

ABSTRACT: Ageing is the most prominent risk for osteoarthritis (OA) development. This study aimed to investigate the role of phosphoinositide-specific phospholipase Cγ (PLCγ) 1, previously linked to OA progression, in regulating age-related changes in articular cartilage and subchondral bone. d-galactose (d-Gal) was employed to treat chondrocytes from rats and mice or injected intraperitoneally into C57BL/6 mice. RTCA, qPCR, Western blot and immunohistochemistry assays were used to evaluate cell proliferation, matrix synthesis, senescence genes and senescence-associated secretory phenotype, along with PLCγ1 expression. Subchondral bone morphology was assessed through micro-CT. In mice with chondrocyte-specific Plcg1 deficiency (Plcg1^flox/flox; Col2a1-CreERT), articular cartilage and subch

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC11332598 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature