Novel 3D human trophoblast culture to explore T. cruzi infection in the placenta.

ABSTRACT:

Introduction

Human trophoblastic cell lines, such as BeWo, are commonly used in 2D models to study placental Trypanosoma cruzi infections. However, these models do not accurately represent natural infections. Three-dimensional (3D) microtissue cultures offer a more physiologically relevant in vitro model, mimicking tissue microarchitecture and providing an environment closer to natural infections. These 3D cultures exhibit functions such as cell proliferation, differentiation, morphogenesis, and gene expression that resemble in vivo conditions.

Methods

We developed a 3D culture model using the human trophoblastic cell line BeWo and nonadherent agarose molds from the MicroTissues® 3D Petri Dish® system. Both small (12-256) and large (12-81) models were tested with vary

SUBMITTER: Apodaca S 

PROVIDER: S-EPMC11333438 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature