Optimizing Archaeal Lipid Biosynthesis in <i>Escherichia coli</i>.

ABSTRACT: Membrane lipid chemistry is remarkably different in archaea compared with bacteria and eukaryotes. In the evolutionary context, this is also termed the lipid divide and is reflected by distinct biosynthetic pathways. Contemporary organisms have almost without exception only one type of membrane lipid. During early membrane evolution, mixed membrane stages likely occurred, and it was hypothesized that the instability of such mixtures was the driving force for the lipid divide. To examine the compatibility between archaeal and bacterial lipids, the bacterium Escherichia coli has been engineered to contain both types of lipids with varying success. Only limited production of archaeal lipid archaetidylethanolamine was achieved. Here, we substantially increased its production in E. co

SUBMITTER: Hoekzema M 

PROVIDER: S-EPMC11334171 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature