Cell-Free Gene Expression in Bioprinted Fluidic Networks.

ABSTRACT: The realization of soft robotic devices with life-like properties requires the engineering of smart, active materials that can respond to environmental cues in similar ways as living cells or organisms. Cell-free expression systems provide an approach for embedding dynamic molecular control into such materials that avoids many of the complexities associated with genuinely living systems. Here, we present a strategy to integrate cell-free protein synthesis within agarose-based hydrogels that can be spatially organized and supplied by a synthetic vasculature. We first utilize an indirect printing approach with a commercial bioprinter and Pluronic F-127 as a fugitive ink to define fluidic channel structures within the hydrogels. We then investigate the impact of the gel matrix on the expressi

SUBMITTER: Bienau A 

PROVIDER: S-EPMC11334185 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature