Integrative in vivo analysis of the ethanolamine utilization bacterial microcompartment in Escherichia coli.

ABSTRACT: Bacterial microcompartments (BMCs) are self-assembling protein megacomplexes that encapsulate metabolic pathways. Although approximately 20% of sequenced bacterial genomes contain operons encoding putative BMCs, few have been thoroughly characterized, nor any in the most studied Escherichia coli strains. We used an interdisciplinary approach to gain deep molecular and functional insights into the ethanolamine utilization (Eut) BMC system encoded by the eut operon in E. coli K-12. The eut genotype was linked with the ethanolamine utilization phenotype using deletion and overexpression mutants. The subcellular dynamics and morphology of the E. coli Eut BMCs were characterized in cellula by fluorescence microscopy and electron (cryo)microscopy. The mi

SUBMITTER: Jallet D 

PROVIDER: S-EPMC11334477 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature