Multiplexed immunolabelling of cancer using bioconjugated plasmonic gold-silver alloy nanoparticles.

ABSTRACT: Reliable protein detection methods are vital for advancing biological research and medical diagnostics. While immunohistochemistry and immunofluorescence are commonly employed, their limitations underscore the necessity for alternative approaches. This study introduces immunoplasmonic labelling, utilizing plasmonic nanoparticles (NPs), specifically designed gold and gold-silver alloy NPs (Au:Ag NPs), for multiplexed and quantitative protein detection. These NPs, when coupled with antibodies targeting proteins of interest, enable accurate counting and evaluation of protein expression levels while overcoming issues such as autofluorescence. In this study, we compare two nanoparticle functionalization strategies-one coating based on thiolated PEG and one coating based on calix[4]arenes-on gol

SUBMITTER: Darviot C 

PROVIDER: S-EPMC11334976 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature