Lactate modulates zygotic genome activation through H3K18 lactylation rather than H3K27 acetylation.

ABSTRACT: In spite of its essential role in culture media, the precise influence of lactate on early mouse embryonic development remains elusive. Previous studies have implicated lactate accumulation in medium affecting histone acetylation. Recent research has underscored lactate-derived histone lactylation as a novel epigenetic modification in diverse cellular processes and diseases. Our investigation demonstrated that the absence of sodium lactate in the medium resulted in a pronounced 2-cell arrest at the late G2 phase in embryos. RNA-seq analysis revealed that the absence of sodium lactate significantly impaired the maternal-to-zygotic transition (MZT), particularly in zygotic gene activation (ZGA). Investigations were conducted employing Cut&Tag assays targeting the well-studied histone acetyla

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC11335220 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature