ZMYND12 serves as an IDAd subunit that is essential for sperm motility in mice.

ABSTRACT: Inner dynein arms (IDAs) are formed from a protein complex that is essential for appropriate flagellar bending and beating. IDA defects have previously been linked to the incidence of asthenozoospermia (AZS) and male infertility. The testes-enriched ZMYND12 protein is homologous with an IDA component identified in Chlamydomonas. ZMYND12 deficiency has previously been tied to infertility in males, yet the underlying mechanism remains uncertain. Here, a CRISPR/Cas9 approach was employed to generate Zmynd12 knockout (Zmynd12^-/-) mice. These Zmynd12^-/- mice exhibited significant male subfertility, reduced sperm motile velocity, and impaired capacitation. Through a combination of co-immunoprecipitation and mass spectrometry, ZMYND12 was found to interact with TTC29 and PRK

SUBMITTER: Wang C 

PROVIDER: S-EPMC11335240 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature