Interactive visualization of nanopore sequencing signal data with Squigualiser.

ABSTRACT:

Motivation

Nanopore sequencing current signal data can be 'basecalled' into sequence information or analysed directly, with the capacity to identify diverse molecular features, such as DNA/RNA base modifications and secondary structures. However, raw signal data is large and complex, and there is a need for improved visualization strategies to facilitate signal analysis, exploration and tool development.

Results

Squigualiser (Squiggle visualiser) is a toolkit for intuitive, interactive visualization of sequence-aligned signal data, which currently supports both DNA and RNA sequencing data from Oxford Nanopore Technologies instruments. Squigualiser is compatible with a wide range of alternative signal-alignment software packages and enables visualization of both signal-to-rea

SUBMITTER: Samarakoon H 

PROVIDER: S-EPMC11335371 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature