Characterization and genomic analysis of Bacillus megaterium with the ability to degrade aflatoxin B₁.

ABSTRACT: Coix seed is a good product for both medicinal and food use, which is highly susceptible to aflatoxin B₁ (AFB₁) contamination during field transport, storage, and processing. The aim of this study is to find microbial strains that can solve the problem of contamination of coix seed. In this study, the AFB₁-degrading microorganism SX1-1 was isolated and identified as a Bacillus megaterium based on morphology, microscopy, and 16S rDNA sequencing. The optimum culture conditions for SX1-1 to degrade AFB₁ were determined to be 12 h. The optimum degradation conditions were 72 h, 57°C, and an initial pH of 8.0. The highest degradation of AFB₁ was observed in the fermentation supernatant of the SX1-1 strain, with a degradation rate of 9

SUBMITTER: Li T 

PROVIDER: S-EPMC11335518 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature