The arrangements of the microvasculature and surrounding glial cells are linked to blood-brain barrier formation in the cerebral cortex.

ABSTRACT: The blood-brain barrier (BBB) blocks harmful substances from entering the brain and dictates the central nervous system (CNS)-specific pharmacokinetics. Recent studies have shown that perivascular astrocytes and microglia also control BBB functions, however, information about the formation of BBB glial architecture remains scarce. We investigated the time course of the formation of BBB glial architecture in the rat brain cerebral cortex using Evans blue (EB) and tissue fixable biotin (Sulfo-NHS Biotin). The extent of the leakage into the brain parenchyma showed that the BBB was not formed at postnatal Day 4 (P4). The BBB gradually strengthened and reached a plateau at P15. We then investigated the changes in the configurations of blood vessels, astrocytes, and microglia with age by 3D imag

SUBMITTER: Shigemoto-Mogami Y 

PROVIDER: S-EPMC11335649 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature