Visualisation of in vivo protein synthesis during mycobacterial infection through [⁶⁸Ga]Ga-DOTA-puromycin µPET/MRI.

ABSTRACT: Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct quantification of puromycin binding. This study assesses the value of [⁶⁸Ga]Ga-DOTA-puromycin in the visualisation of mycobacteria through positron emission tomography combined with magnetic resonance imaging (µPET/MRI). The radiopharmaceutical was produced by previously published and validated methods. [⁶⁸Ga]Ga-DOTA-Puromycin imaging was performed on severe immunodeficient mice infected with Bacille Calmette-Guérin-derived M. Bovis (BCG). Acute and chronic infection stages were examined by µPET/MRI. A follow-up group o

SUBMITTER: Eigner S 

PROVIDER: S-EPMC11335739 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature