Extracellular vesicles may provide an alternative detoxification pathway during skeletal muscle myoblast ageing.

ABSTRACT: Skeletal muscle (SM) acts as a secretory organ, capable of releasing myokines and extracellular vesicles (SM-EVs) that impact myogenesis and homeostasis. While age-related changes have been previously reported in murine SM-EVs, no study has comprehensively profiled SM-EV in human models. To this end, we provide the first comprehensive comparison of SM-EVs from young and old human primary skeletal muscle cells (HPMCs) to map changes associated with SM ageing. HPMCs, isolated from young (24 ± 1.7 years old) and older (69 ± 2.6 years old) participants, were immunomagnetically sorted based on the presence of the myogenic marker CD56 (N-CAM) and cultured as pure (100% CD56⁺) or mixed populations (MP: 90% CD56⁺). SM-EVs were isolated using an optimised protocol combining ul

SUBMITTER: Fernandez-Rhodes M 

PROVIDER: S-EPMC11336379 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature