Screening chondrocyte necroptosis-related genes in the diagnosis and treatment of osteoarthritis.

ABSTRACT:

Background

Osteoarthritis (OA) is the most common form of joint diseases, with hallmark of cartilage degeneration. Recent studies have shown that the pathogenesis of OA is associated with chondrocyte necroptosis.

Methods

In this study, we used single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing data to analyze necroptosis regulation in OA chondrocytes. We performed enrichment analysis, carried out experimental validation, constructed machine learning models, and docked drug molecules.

Results

After least absolute shrinkage and selection operator (LASSO) algorithm screening, 4 hub genes (RIPK3, CYBB, HSP90AB1, and TRAF5) with diagnostic characteristics were obtained. Following the comparison of multiple models, the Bayesian model with an average area under c

SUBMITTER: Deng M 

PROVIDER: S-EPMC11336430 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature