Optimization of 1,3-propanediol production from fermentation of crude glycerol by immobilized Bacillus pumilus.

ABSTRACT: This study investigates the application of crude glycerol to the production of 1,3-propanediol by immobilized cells of Bacillus pumilus. This is a novel application of a naturally occurring producer obtained from a wastewater storage pond in Thailand. Crude glycerol was obtained through the methanolysis of palm oil, which was catalyzed using rice bran lipase. Ten components of the fermentation medium were screened using a Plackett-Burman design. The statistical significance of the results was determined using multiple linear regression with a backward elimination approach. The significance level was set to 5 % (p < 0.05). Only crude glycerol, (NH₄)₂SO₄, MgSO₄, and CaCl₂ significantly affected 1,3-propanediol production by

SUBMITTER: Pinyaphong P 

PROVIDER: S-EPMC11336579 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature