Nicotinamide riboside Induced Energy Stress and Metabolic Reprogramming in BEAS-2B Cells.

ABSTRACT: Nicotinamide riboside (NR), a NAD⁺ precursor, has received attention due to several health benefits it has induced in experimental models. Studies in cultured cells, animals, and humans consistently show increased NAD⁺ availability after NR supplementation, which is considered the only mode of NR action that leads to health benefits. In the present study, we show that a persistently low NR concentration (1 μM) in the growth medium of BEAS-2B human cells, grown in a monolayer, induces energy stress, which precedes a cellular NAD⁺ increase after 192 h. NR concentrations greater than 1 μM under the specified conditions were cytotoxic in the 2D cell culture model, while all concentrations tested in the 3D cell culture model (BEAS-2B cell spheroids exposed to 1,

SUBMITTER: Cordeiro EWF 

PROVIDER: S-EPMC11337214 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature