CLDN6 inhibits breast cancer growth and metastasis through SREBP1-mediated RAS palmitoylation.

ABSTRACT:

Background

Breast cancer (BC) ranks as the third most fatal malignant tumor worldwide, with a strong reliance on fatty acid metabolism. CLDN6, a candidate BC suppressor gene, was previously identified as a regulator of fatty acid biosynthesis; however, the underlying mechanism remains elusive. In this research, we aim to clarify the specific mechanism through which CLDN6 modulates fatty acid anabolism and its impact on BC growth and metastasis.

Methods

Cell function assays, tumor xenograft mouse models, and lung metastasis mouse models were conducted to evaluate BC growth and metastasis. Human palmitic acid assay, triglyceride assay, Nile red staining, and oil red O staining were employed to investigate fatty acid anabolism. Reverse transcription polymerase chain reaction (R

SUBMITTER: Jin Q 

PROVIDER: S-EPMC11337767 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature