Med3-mediated NADPH generation to help <i>Saccharomyces cerevisiae</i> tolerate hyperosmotic stress.

ABSTRACT: Hyperosmotic stress tolerance is crucial for Saccharomyces cerevisiae in producing value-added products from renewable feedstock. The limited understanding of its tolerance mechanism has impeded the application of these microbial cell factories. Previous studies have shown that Med3 plays a role in hyperosmotic stress in S. cerevisiae. However, the specific function of Med3 in hyperosmotic stress tolerance remains unclear. In this study, we showed that the deletion of the mediator Med3 impairs S. cerevisiae growth under hyperosmotic stress. Phenotypic analyses and yeast two-hybrid assays revealed that Med3 interacts with the transcription factor Stb5 to regulate the expression of the genes gnd1 and ald6, which are involved in NADPH production under hypero

SUBMITTER: Hou S 

PROVIDER: S-EPMC11337799 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature