Archaeal mevalonate pathway in the uncultured bacterium <i>Candidatus</i> Promineifilum breve belonging to the phylum Chloroflexota.

ABSTRACT: The archaeal mevalonate pathway is a recently discovered modified version of the eukaryotic mevalonate pathway. This pathway is widely conserved in archaea, except for some archaeal lineages possessing the eukaryotic or other modified mevalonate pathways. Although the pathway seems almost exclusive to the domain Archaea, the whole set of homologous genes of the pathway is found in the metagenome-assembled genome sequence of an uncultivated bacterium, Candidatus Promineifilum breve, of the phylum Chloroflexota. To prove the existence of the archaea-specific pathway in the domain Bacteria, we confirmed the activities of the enzymes specific to the pathway, phosphomevalonate dehydratase and anhydromevalonate phosphate decarboxylase, because only these two enzymes are absent in closely

SUBMITTER: Kanno K 

PROVIDER: S-EPMC11337835 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature