Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions.

ABSTRACT: Local mRNA translation in axons is critical for the spatiotemporal regulation of the axonal proteome. A wide variety of mRNAs are localized and translated in axons; however, how protein synthesis is regulated at specific subcellular sites in axons remains unclear. Here, we establish that the axonal endoplasmic reticulum (ER) supports axonal translation in developing rat hippocampal cultured neurons. Axonal ER tubule disruption impairs local translation and ribosome distribution. Using nanoscale resolution imaging, we find that ribosomes make frequent contacts with axonal ER tubules in a translation-dependent manner and are influenced by specific extrinsic cues. We identify P180/RRBP1 as an axonally distributed ribosome receptor that regulates local translation and binds to mRNAs enriched f

SUBMITTER: Koppers M 

PROVIDER: S-EPMC11338522 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature