Targeting PLOD2 suppresses invasion and metastatic potential in radiorecurrent prostate cancer.

ABSTRACT:

Background

Metastatic relapse of prostate cancer after radiotherapy is a significant cause of prostate cancer-related morbidity and mortality. PLOD2 is a mediator of invasion and metastasis that we identified as being upregulated in our highly aggressive radiorecurrent prostate cancer cell line.

Methods

Patient dataset analysis was conducted using a variety of prostate cancer cohorts. Prostate cancer cell lines were treated with siRNA, or the drug PX-478 prior to in vitro invasion, migration, or in vivo chick embryo (CAM) extravasation assay. Protein levels were detected by western blot. For RNA analysis, RNA sequencing was conducted on PLOD2 knockdown cells and validated by qRT-PCR.

Results

PLOD2 is a negative prognostic factor associated with biochemical relapse, dr

SUBMITTER: Frame G 

PROVIDER: S-EPMC11338830 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature