Gene expression analysis in mitochondria from chagasic mice: alterations in specific metabolic pathways.

ABSTRACT: Cardiac hypertrophy and remodelling in chagasic disease might be associated with mitochondrial dysfunction. In the present study, we characterized the cardiac metabolic responses to Trypanosoma cruzi infection and progressive disease severity using a custom-designed mitoarray (mitochondrial function-related gene array). Mitoarrays consisting of known, well-characterized mitochondrial function-related cDNAs were hybridized with 32P-labelled cDNA probes generated from the myocardium of mice during immediate early, acute and chronic phases of infection and disease development. The mitoarray successfully identified novel aspects of the T. cruzi-induced alterations in the expression of the genes related to mitochondrial function and biogenesis that were further confirmed by real-time reverse tr

SUBMITTER: Garg N 

PROVIDER: S-EPMC1133884 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature