Identification of miRNAs related to osteoporosis by high-throughput sequencing.

ABSTRACT:

Background

Osteoporosis is a major health issue. MicroRNAs (miRNAs) play multiple roles in regulating cell growth and development. High-throughput sequencing technology is widely used nowadays.

Objective

To screen for and validate miRNAs associated with osteoporosis.

Method

Bone specimens from patients with (n = 3) and without (n = 3) osteoporosis were collected. High-throughput sequencing was used to screen for miRNAs that were then analyzed using volcano maps, Wayne maps, gene ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Confirmation of the miRNAs was done using qRT-PCR.

Results

The analysis of sequencing showed that there were 12 miRNAs that were down-regulated and five miRNAs that were upregulated in osteoporosis. GO

SUBMITTER: Huang J 

PROVIDER: S-EPMC11338934 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature