Comparison, optimization and antioxidant activity of ultrasound-assisted natural deep eutectic solvents extraction and traditional method: A greener route for extraction of flavonoid from Moringa oleifera Lam. leaves.

ABSTRACT: To develop an environmentally sustainable and efficient extraction method for flavonoids from Moringa oleifera Lam. (M. oleifera) leaves, natural deep eutectic solvents (NADES) with ultrasound-assisted extraction was utilized in this study. After optimization of extraction parameters of NADES, including ultrasonic power, ultrasonic time, and liquid-solid ratio, the extraction yield of ultrasound-assisted NADES (UAN) composed of betaine and urea (Bet-Urea) reached 54.69 ± 0.19 mg RE/g DW, which made a 1.7-fold increase compared to traditional ultrasound-assisted traditional solvent (UATS). UPLC-Q Exactive/MS analysis revealed that M. oleifera leaves flavonoids (MOLF) was mainly composed of Quercetin 3-β-D-glucoside, Rutin, Kaempferol-3-O-glucoside, Vitexin and Quercetin. Furthermore, the CO

SUBMITTER: Peng W 

PROVIDER: S-EPMC11339064 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature