Kinetic characterization of two neuraminic acid synthases and evaluation of their application potential.

ABSTRACT: Neuraminic acid synthases are an important yet underexplored group of enzymes. Thus, in this research, we performed a detailed kinetic and stability analysis and a comparison of previously known neuraminic acid synthase from Neisseria meningitidis, and a novel enzyme, PNH₅, obtained from a metagenomic library. A systematic analysis revealed a high level of similarity of PNH₅ to other known neuraminic acid synthases, except for its pH optimum, which was found to be at 5.5 for the novel enzyme. This is the first reported enzyme from this family that prefers an acidic pH value. The effect of different metal cofactors on enzyme activity, i.e. Co²⁺, Mn²⁺ and Mg²⁺, was studied systematically. The kinetics of neuraminic acid synthesis was com

SUBMITTER: Cakar MM 

PROVIDER: S-EPMC11339185 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature