Bimetallic Single-Atom Nanozyme-Based Electrochemical-Photothermal Dual-Function Portable Immunoassay with Smartphone Imaging.

ABSTRACT: Rapid and accurate detection of human epidermal growth factor receptor 2 (HER2) is crucial for the early diagnosis and prognosis of breast cancer. In this study, we reported an iron-manganese ion N-doped carbon single-atom catalyst (FeMn-NC_etch/SAC) bimetallic peroxidase mimetic enzyme with abundant active sites etched by H₂O₂ and further demonstrated unique advantages of single-atom bimetallic nanozymes in generating hydroxyl radicals by density functional theory (DFT) calculations. As a proof of concept, a portable device-dependent electrochemical-photothermal bifunctional immunoassay detection platform was designed to achieve reliable detection of HER2. In the enzyme-linked reaction, H₂O₂ was generated by substrate catalysis via sec

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11339724 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature