Engineering a non-oxidative glycolysis pathway in escherichia coli for high-level citramalate production.

ABSTRACT:

Background

Methyl methacrylate (MMA) is a key precursor of polymethyl methacrylate, extensively used as a transparent thermoplastic in various industries. Conventional MMA production poses health and environmental risks; hence, citramalate serves as an alternative bacterial compound precursor for MMA production. The highest citramalate titer was previously achieved by Escherichia coli BW25113. However, studies on further improving citramalate production through metabolic engineering are limited, and phage contamination is a persistent problem in E. coli fermentation.

Results

This study aimed to construct a phage-resistant E. coli BW25113 strain capable of producing high citramalate titers from glucose. First, promoters and heterologous cimA genes were screened, and an effect

SUBMITTER: Wang T 

PROVIDER: S-EPMC11340173 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature