Characterization of three novel members of the Arabidopsis thaliana equilibrative nucleoside transporter (ENT) family.

ABSTRACT: Research on metabolism of nucleotides and their derivatives has gained increasing interest in the recent past. This includes de novo synthesis, analysis of salvage pathways, breakdown and transport of nucleotides, nucleosides and nucleobases. To perform a further step towards the analysis of nucleoside transport in Arabidopsis, we incubated leaf discs with various radioactively labelled nucleosides. Leaf cells imported labelled nucleosides and incorporated these compounds into RNA, but not into DNA. Furthermore, we report on the biochemical properties of three so far uncharacterized members of the Arabidopsis ENT (equilibrative nucleoside transporter) family (AtENT4, AtENT6 and AtENT7). After heterologous expression in yeast, all three proteins exhibited broad substrate specificity and tra

SUBMITTER: Wormit A 

PROVIDER: S-EPMC1134039 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature