Signal interference between drugs and metabolites in LC-ESI-MS quantitative analysis and its evaluation strategy.

ABSTRACT: Liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS) is a widely utilized technique for in vivo pharmaceutical analysis. Ionization interference within electrospray ion source, occurring between drugs and metabolites, can lead to signal variations, potentially compromising quantitative accuracy. Currently, method validation often overlooks this type of signal interference, which may result in systematic errors in quantitative results without matrix-matched calibration. In this study, we conducted an investigation using ten different groups of drugs and their corresponding metabolites across three LC-ESI-MS systems to assess the prevalence of signal interference. Such interferences can potentially cause or enhance nonlinearity in the calibration curves of drugs

SUBMITTER: Jiang F 

PROVIDER: S-EPMC11340582 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature