Tanycytic transcytosis inhibition disrupts energy balance, glucose homeostasis and cognitive function in male mice.

ABSTRACT:

Objectives

In Western society, high-caloric diets rich in fats and sugars have fueled the obesity epidemic and its related disorders. Disruption of the body-brain communication, crucial for maintaining glucose and energy homeostasis, arises from both obesogenic and genetic factors, leading to metabolic disorders. Here, we investigate the role of hypothalamic tanycyte shuttles between the pituitary portal blood and the third ventricle cerebrospinal fluid in regulating energy balance.

Methods

We inhibited vesicle-associated membrane proteins (VAMP1-3)-mediated release in tanycytes by expressing the botulinum neurotoxin type B light chain (BoNT/B) in a Cre-dependent manner in tanycytes. This was achieved by injecting either TAT-Cre in the third ventricle or an AAV1/2 expressing

SUBMITTER: Duquenne M 

PROVIDER: S-EPMC11340606 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature