Targeting Gsk3a reverses immune evasion to enhance immunotherapy in hepatocellular carcinoma.

ABSTRACT: Immune escape is an important feature of hepatocellular carcinoma (HCC). The overall response rate of immune checkpoint inhibitors (ICIs) in HCC is still limited. Revealing the immune regulation mechanisms and finding new immune targets are expected to further improve the efficacy of immunotherapy. Our study aims to use CRISPR screening mice models to identify potential targets that play a critical role in HCC immune evasion and further explore their value in improving immunotherapy. We performed CRISPR screening in two mice models with different immune backgrounds (C57BL/6 and NPG mice) and identified the immunosuppressive gene Gsk3a as a candidate for further investigation. Flow cytometry was used to analyze the impact of Gsk3a on immune cell infiltration and T-cell function. RNA sequenc

SUBMITTER: Zheng X 

PROVIDER: S-EPMC11340705 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature