Infection of human organoids supports an intestinal niche for Chlamydia trachomatis.

ABSTRACT: Several reports suggest that intestinal tissue may be a natural niche for Chlamydia trachomatis infection and a reservoir for persistent infections in the human body. Due to the human specificity of the pathogen and the lack of suitable host models, there is limited knowledge on this topic. In our study, we modelled the course of the chlamydial infection in human primary gastrointestinal (GI) epithelial cells originating from patient-derived organoids. We show that GI cells are resistant to apical infection and C. trachomatis needs access to the basolateral membrane to establish an infection. Transmission electron microscopy analysis reveals the presence of both normal as well as aberrant chlamydial developmental forms in the infected cells, suggesting a possible cell-type specific nature

SUBMITTER: Hovhannisyan P 

PROVIDER: S-EPMC11340892 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature