OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.

ABSTRACT: Most essential cellular functions are performed by proteins assembled into larger complexes. Fluorescence Polarization Microscopy (FPM) is a powerful technique that goes beyond traditional imaging methods by allowing researchers to measure not only the localization of proteins within cells, but also their orientation or alignment within complexes or cellular structures. FPM can be easily integrated into standard widefield microscopes with the addition of a polarization modulator. However, the extensive image processing and analysis required to interpret the data have limited its widespread adoption. To overcome these challenges and enhance accessibility, we introduce OOPS (Object-Oriented Polarization Software), a MATLAB package for object-based analysis of FPM data. By combining flexible

SUBMITTER: Dean WF 

PROVIDER: S-EPMC11341096 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature