Characteristics and impact of infiltration of B-cells from systemic sclerosis patients in a 3D healthy skin model.

ABSTRACT:

Introduction

In systemic sclerosis (SSc), B-cells are activated and present in the skin and lung of patients where they can interact with fibroblasts. The precise impact and mechanisms of the interaction of B-cells and fibroblasts at the tissular level are poorly studied.

Objective

We investigated the impact and mechanisms of B-cell/fibroblast interactions in cocultures between B-cells from patients with SSc and 3-dimensional reconstituted healthy skin model including fibroblasts, keratinocytes and extracellular matrix.

Methods

The quantification and description of the B-cell infiltration in 3D cocultures were performed using cells imagery strategy and cytometry. The effect of coculture on the transcriptome of B-cells and fibroblasts was studied with bulk and single-c

SUBMITTER: Le Maitre M 

PROVIDER: S-EPMC11341436 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature