Effects of tumor treating fields (TTFields) on human mesenchymal stromal cells.

ABSTRACT:

Purpose

Mesenchymal stromal cells (MSCs) within the glioblastoma microenvironment have been shown to promote tumor progression. Tumor Treating Fields (TTFields) are alternating electric fields with low intensity and intermediate frequency that exhibit anti-tumorigenic effects. While the effects of TTFields on glioblastoma cells have been studied previously, nothing is known about the influence of TTFields on MSCs.

Methods

Single-cell RNA sequencing and immunofluorescence staining were employed to identify glioblastoma-associated MSCs in patient samples. Proliferation and clonogenic survival of human bone marrow-derived MSCs were assessed after TTFields in vitro. MSC' characteristic surface marker expression was determined using flow cytometry, while multi-lineage differentia

SUBMITTER: Strack M 

PROVIDER: S-EPMC11341748 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature