Atelocollagen supports three-dimensional culture of human induced pluripotent stem cells.

ABSTRACT: As autologous induced pluripotent stem cell (iPSC) therapy requires a custom-made small-lot cell production line, and the cell production method differs significantly from the existing processes for producing allogeneic iPSC stocks for clinical use. Specifically, mass culture to produce stock is no longer necessary; instead, a series of operations from iPSC production to induction of differentiation of therapeutic cells must be performed continuously. A three-dimensional (3D) culture method using small, closed-cell manufacturing devices is suitable for autologous iPSC therapy. The use of such devices avoids the need to handle many patient-derived specimens in a single clean room; handling of cell cultures in an open system in a cell processing facility increases the risk of infection. In t

SUBMITTER: Nakashima Y 

PROVIDER: S-EPMC11342089 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature