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A multi-nutrient array protocol to study disease-diet interactions in Drosophila melanogaster.

ABSTRACT: The availability of a defined fully synthetic diet for the fruit fly Drosophila melanogaster allows for complete and precise manipulation of its nutritional environment. Here, we present a protocol for performing large-scale multivariate nutrient analysis via the traditional diet preparation approach, or by adding nutrient solutions to a baseline medium. We detail procedures from sample collection to data analysis. This protocol has applications for the study of nutrition-life trait interactions and nutrigenomics, to reveal interactions between genotype and diet composition. For complete details on the use and execution of this protocol, please refer to Martelli et al.¹ and Martelli et al.².

SUBMITTER: Lin J

PROVIDER: S-EPMC11342171 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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A multi-nutrient array protocol to study disease-diet interactions in Drosophila melanogaster.

Lin Jiayi J Martelli Felipe F Mele Sarah S Quig Annelise A Johnson Travis K TK Piper Matthew D W MDW

STAR protocols 20240731 3

The availability of a defined fully synthetic diet for the fruit fly Drosophila melanogaster allows for complete and precise manipulation of its nutritional environment. Here, we present a protocol for performing large-scale multivariate nutrient analysis via the traditional diet preparation approach, or by adding nutrient solutions to a baseline medium. We detail procedures from sample collection to data analysis. This protocol has applications for the study of nutrition-life trait interactions ...[more]

PMID: 39083384

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