A Multiplex HIV Incidence Assay for Inferring Recent HIV-1 Transmission and Time of Infection.

ABSTRACT:

Background

Laboratory assays for determining recent HIV-1 infection are an important public health tool for aiding in the estimation of HIV incidence. Some incidence assay analytes are remarkably predictive of time since seroconversion and may be useful for additional applications, such as predicting recent transmission events during HIV outbreaks and informing prevention strategies.

Methods

Plasma samples (n = 154) from a recent HIV-1 outbreak in a rural community in Indiana were tested with the customized HIV-1 Multiplex assay, based on the Bio-Rad Bio-Plex platform, which measures antibody response to HIV envelope antigens, gp120, gp160, and gp41. Assay cutoffs for each analyte were established to determine whether an individual seroconverted within 30, 60, or 90 days of

SUBMITTER: Curtis KA 

PROVIDER: S-EPMC11342239 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature