MiR-34b promotes oxidative stress and induces cellular senescence through TWIST1 in human cervical cancer.

ABSTRACT:

Purpose

The aim of this research was to elucidate the role of miR-34b in cervical cancer progression and the underlying mechanism behind the miR-34b-mediated tumor suppression. The study revealed the role of miR-34b as a senescence inducer and serves as a potential therapeutic target in developing combination therapy with senotherapeutics.

Methods

MiR-34b was ectopically expressed in cervical cancer cell lines using a tetracycline inducible system and its effects on cell viability, apoptosis, senescence, DNA damage and oxidative stress were studied using MTT assay, acridine orange/ ethidium bromide staining, senescence associated β-galactosidase assay, gamma H2AX foci staining assay, western blotting and specific dyes for the detection of total and individual ROS species.

SUBMITTER: Sindhu KJ 

PROVIDER: S-EPMC11342277 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature